Production of Human Lymphoblastoid Interferon
نویسندگان
چکیده
منابع مشابه
Production of human lymphoblastoid interferon by Namalva cells.
Optimum conditions for growth and interferon production by a human lymphoblastoid cell line, Namalva, have been studied. Adaptation to large-scale production is possible utilizing either Sendai virus or Newcastle disease virus. Priming of cultures before induction is unnecessary. The interferon produced has properties similar to human leukocyte interferon. The production of lymphoblastoid inter...
متن کاملHuman lymphoblastoid interferon. Large scale production and partial purification.
Human lymphoblastoid interferon was produced on an 800-liter scale (2.6 X 10(9) units) by induction of Namalva cells with Newcastle disease virus, strain B1. The interferon was partially purified by anti-leukocyte interferon affinity chromatography, sulfopropyl Sephadex ion exchange chromatography, isoelectric focusing, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Recovery of ...
متن کاملSpontaneous production of gamma interferon and induced production of beta interferon by human T-lymphoblastoid cell lines.
Two human T-lymphoblastoid cell lines, CCRF/CEM and Molt 4, produced beta interferon (IFN-beta) upon infection with Sendai virus. Molt 4, but not CCRF/CEM, spontaneously produced up to 300 U of IFN-gamma per ml, apparently not contaminated with IFN-alpha or -beta. Phytohemagglutinin, a T-cell mitogen, did not stimulate IFN production in these lines. A third T-lymphoblastoid line, CCRF/HSB2, pro...
متن کاملInterferon production by a human lymphoblastoid cell line (DG-75) free of the Epstein-Barr genome.
A new lymphoblastoid cell line, DG-75, was investigated for its ability to produce interferon. DG-75 cells, previously shown to be free of Epstein-Barr virus genome and receptors, could be grown in submerged culture and could produce interferon in titers comparable to interferon produced by Namalva cells. The interferon produced was similar in size to the Namalva interferon as determined by gel...
متن کاملInteraction between human lymphoblastoid interferon and chemotherapeutic agents in vitro.
The combined effect of human lymphoblastoid interferon (HLBI) and anticancer agents on the growth of MOLT-4 was studied in vitro. The interferon showed a strikingly synergistic interaction in combination with aclarubicin, cytosine arabinoside or prednisolone. It was moderately synergistic in combination with adriamycin or 5-fluorouracil and tended to show additive effects with daunorubicin or v...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1975
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.1.1.116-117.1975